Composite
Part:BBa_K200025
Designed by: Royah Vaezi Group: iGEM09_Imperial College London (2009-10-15)
pCstA+RBS+RcsB+TT
This is the functional transcriptional unit which allows expression of the RcsB receiver protein and positive regulation of colanic acid production.
The PcstA promoter is cAMP activated. Under low glucose concentrations, there is increased activity by adenylate cyclase. This results in cAMP binding to the cAMP receptor protein, and activating the promoter for downstream expression of RcsB.
Usage and Biology
This composite part, comprising of subparts BBa_K200020 and BBa_K200024, was used in [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project by Imperial iGEM 2009.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |